Serological Grouping of Hemolytic Streptococci by Counter-Immunoelectrophoresis
نویسندگان
چکیده
منابع مشابه
Serological grouping of hemolytic streptococci by counter-immunoelectrophoresis.
The counter-immunoelectrophoresis (CIE) method of grouping streptococci was more sensitive than the capillary precipitin method. The precipitate was easier to read, and the test was simple to perform and required fewer reagents. An autoclave-Pronase-B extraction procedure is described and was found superior to either acid, Pronase-B, or the autoclave extraction procedures for recovering groupab...
متن کاملSimplified extraction procedure for serological grouping of beta-hemolytic streptococci.
An adaptation of the nitrous acid extraction of streptococci proved to be a reliable and practical method for the preparation of extracts for routine serological group identification. The extracts of all groups tested gave strong capillary precipitin reactions as well as reactions of double diffusion in gel. For routine grouping, extracts were prepared from the first one-half-plate subculture o...
متن کاملSimplified coagglutination test for serological grouping of beta-hemolytic streptococci.
A new coagglutination kit consisting of a plastic slide with dried antibody-coated staphylococci was evaluated for the grouping of beta-hemolytic streptococci of groups A, B, C, and G. The test was compared with the classical precipitation test, using hot formamide antigen extracts with 224 strains of groups A, B, C, and G streptococci. An agreement of 100% was found between the new coagglutina...
متن کاملSerological grouping of streptococci by slide agglutination.
Streptococcal grouping sera for groups A, B, C, and G prepared for conventional testing by precipitation were made specific by absorption and used to identify streptococci by slide agglutination with and without staphyloccocal coagglutination. Trypsinised suspensions of 1055 strains, identified by precipitation as belonging to group A, B, C, or G, were tested by slide agglutination. Of these, 9...
متن کاملThe Serological Typing of Hemolytic Streptococci
The relationships among the hemolytic streptococci have been greatly clarified by the fundamental work of Lancefield (1). She has shown that these organisms may be classified into groups on the basis of a precipitin reaction and ample evidence has accumulated (2, 3) to show that almost all human infections are caused by members of her Group A. Strains of the groups from B to K are responsible f...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1973
ISSN: 0003-6919
DOI: 10.1128/am.26.6.899-903.1973